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Old 05-17-2011, 04:21 AM
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Unhappy Mold in flask

Today I did see something white on some roots of my little Asco's. Yesterday there was nothing wrong. Can this be mold? If so what should I do? Do I have to take them out of the flask? Who can help me? I don't want to loose the seedlings. The problem seems to be only on the roots that grow out of the agar.
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Old 05-17-2011, 05:24 AM
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Photo?
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Old 05-17-2011, 06:45 AM
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Sometimes a grayish mold like you see that grows on old bread you forgot to throw out can get into the flask.
Usually it does no major damage and I would not worry to much unless it starts to grow all over the plants.
The main one to worry about is the so call "wet mold", this is very bad and unless you get the plants out quickly and fungicide them, they are gone.
so a photo close up would help but do not panic yet.
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Old 05-17-2011, 06:48 AM
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If it is fungus it needs to be deflasked before you lose the whole thing.

After you deflask you will have to treat all plants with a fungicide and continue to watch the seedlings to make sure it doesn't pop up again.

Brooke
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Old 05-17-2011, 08:08 AM
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I don't have a camera at this moment. The spots are not growing. They are very tiny, and only on the roots that peek out of the agar. Can it be dried agar. The spots look very dry.
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Old 05-17-2011, 09:26 AM
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are the spots all the same size, and regularly spaced? they might be a natural part of the root. keep checking on it. it might also be mold - if it's only been one day, you might not notice the growth.
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Old 05-17-2011, 09:46 AM
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There are about 3 spots. They are all tiny, but 2 of them are so tiny that you hardly notice them at all.They look a little dried.
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Old 05-17-2011, 09:59 AM
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It sounds like root hairs rather than fungus. Orchids in flask can produce patches of fine hairs on their roots, similar to the plant in photo below. The plant in the photo is a terrestrial orchid, which tend to be a lot hairier than epiphytes, but you sometimes get a similar kind of fuzz on the exposed roots of epiphytes in flask. Fungus, on the other hand will usually spread across the media rather stay on the roots. By all means keep an eye on it but I think you should be fine.


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Old 05-17-2011, 10:05 AM
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I does look like the photo a little, but the spots are more tiny. and less hairy. Also there is nothing to see on the media at all. So I keep a close eye on them. I didn't now it could also possibly be root hairs. Thanks for the info.
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Old 05-17-2011, 07:37 PM
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You description sounds pretty much what I'd expect from an epiphyte in flask compared to the much more hairy Thelymitra in the photo. I don't have any epiphytes in flask at the moment that are beyond the protocorm stage so I can't show you a more relevant picture. The hairs in the photo are less than 1 mm long but in epiphytes they tend to look more more like down and the patches range from small dots to a few mm in length. It seems to occur where the roots aren't in regular contact with moisture.

The fact that there is nothing on the media is a really good argument against it being fungal. Fungi get the same nutrients from culture media as they would from a plant root without any of the hard work of breaking into the root cells and catabolising complex molecules inside into something they can use. I haven't come across any fungal contaminant in flask that preferentially grows on the roots and avoids the media; even mycorrhyzal fungi grow on the media.
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Old 05-18-2011, 01:55 AM
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O.k Thanks a lot. I keep an eye on them, but I guess everything is allright.
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Old 05-23-2011, 03:44 PM
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Thank you for already asking this question and everybody for the tips & answers! I recently got a flask of Psygmorchis pusilla for free at an orchid show from a vendor who was going to throw it out because of bread mold. The vendor recomended diluded dishsoap until I could track down 1) one of two pesticides he recommended - which I don't have the names in front of me at the moment & 2) tracking down tree fern plaque which I should have bought at the orchid show but overlooked! I got much of the mold out and the plants seem to be healthy, two are still in bloom.
Should I just take it out of the agar completely and temporarily pot it in crushed orchid mix until the tree fern mount arrives?
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Old 05-24-2011, 11:52 PM
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Art Geek Mark,
I don't grow Psygmorchis pusilla but seeing as though no one else has responded, I usually deflask into pots regardless of whether I'm intending to mount the orchid in the future. I only deflask directly on to mounts when the orchid in question is intolerant of pot culture (eg many twig epiphytes). Given that Google Images pulls up several photo's of Psygmorchis pusilla in pots, I doubt this applies to your orchid.
Andrew
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Old 05-30-2011, 12:26 PM
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Thank you Andrew I noticed that in photos while I was googling as well. I think I will pot them ASAP since the fern plaque is taking it's merry time being shipped! Thanks again for the advice.
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