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  #76 (permalink)  
Old 03-04-2009, 10:54 AM
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It works well for both just use 2 bananas instead of 1 for the replate.
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  #77 (permalink)  
Old 03-08-2009, 10:09 AM
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Ahhhhhh thanks...and if I had read your recipe more thoroughly, I would have known the answer!! Thanks!
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  #78 (permalink)  
Old 03-08-2009, 10:32 AM
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Doodle..The babies are looking great! I almost wet myself when I saw the last pictures! This is so cool...
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Old 03-08-2009, 01:46 PM
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Ok now get a hold of yourself Patticake!!!! Do you need a "Depends"?? LOL. Yes they're growing up fast and I reflasked the remaining mother flasks...so I have baby dends coming out of my ears. I never thought I would ever get to the point where I would ask this question but here goes....how long before they'll have to be replated again....OR do they stay in this flask for the duration (however long THAT is)???
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Old 03-08-2009, 02:08 PM
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Quote:
Originally Posted by doodlekitty View Post
Ok now get a hold of yourself Patticake!!!! Do you need a "Depends"?? LOL. Yes they're growing up fast and I reflasked the remaining mother flasks...so I have baby dends coming out of my ears. I never thought I would ever get to the point where I would ask this question but here goes....how long before they'll have to be replated again....OR do they stay in this flask for the duration (however long THAT is)???
I work in a retirement home for nuns. I may just have to get a box of those!

Everything that I've read, says that this is the last (and only) re-plate. I know that when I was shopping for a flasking lab last year, they all charged for initial flaskings and however many flasks you wanted from the re-plate. I believe that you just let 'em grow from here! The one flask that I bought and de-flasked myself, is a success so far. The smallest seedlings didn't make it, but I was told 24 plants- and I've got about 29 that made it. I wouldn't de-flask too early, that's for sure. I would sure love to try flasking, but it makes me nervous.

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Old 03-08-2009, 05:10 PM
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She is correct, you should not need to reflask though there is no reason you could not if you wanted to get a special cross bigger before they come out of the flask. Just grow them until they are big enough to grow in a compot or a tray.

I have seen people grow a small number (2-5) in a tall stand up flask until they reached the top of the flask. This allows them to go strait into a 2 inch pot.

At one time I had a miniture Oncidium species that I bloomed in a lay down flask. It was always in bloom and was a great conversation piece, I kept it that way for about 2 years.
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  #82 (permalink)  
Old 03-11-2009, 06:49 PM
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What a great thread! Doodlekitty, you rock. Nothing like sterile, scientific procedures to get me excited. Wooo Hooo!
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  #83 (permalink)  
Old 03-11-2009, 10:42 PM
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Why thank you Plucky....and for your viewing pleasure you can checkout the thread above this one whereby I freak out about brown roots....
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  #84 (permalink)  
Old 05-07-2009, 11:37 AM
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Baby orchid update!!

Ok let me try this again....

Here are some pics of the "chids"!! I'm thinking its time to get them outta there........can I get some advice from the experts???
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File Type: jpg Dends 5-07-09.jpg (56.2 KB, 69 views)
File Type: jpg Dend replates 5-07-09.jpg (58.7 KB, 62 views)
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  #85 (permalink)  
Old 05-07-2009, 11:51 AM
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Quote:
Originally Posted by doodlekitty View Post
Ok let me try this again....

Here are some pics of the "chids"!! I'm thinking its time to get them outta there........can I get some advice from the experts???
They look great! Makes me really wanna try it myself. I'll have to reread this thread afew times. Hmmm....I've got two Phals' in blooms (Phal. Fireberry 'Orchidheights' and a noid yellow w/spotting/barring). Maybe I should for practice......... 7 months for a Phal. Then again I'd have my Catt. flasks back before these would be ready. Does anyone have any capsules growing? This would make for an interested geek project for us all. Thanks for the inspiration doodlekitty.




Last edited by exasperatus2002; 05-07-2009 at 11:59 AM.
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  #86 (permalink)  
Old 05-07-2009, 12:01 PM
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Very impressive what I am reading here, the progress is amazing! Very well done...
I am just about speachless I got to give this a try!
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  #87 (permalink)  
Old 05-07-2009, 03:07 PM
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They are doing so well!

They do look like the are ready for the next stage, whatever that is. I hope it keeps progressing smoothly!
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Old 05-07-2009, 03:16 PM
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Great growing Doodlekitty! I love this thread also! Keep us posted!!
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  #89 (permalink)  
Old 05-07-2009, 06:11 PM
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Thank you everyone!! Your encouragement is very much appreciated!!! As I read back thru the thread it would appear that I'm just a LITTLE anxious to get those puppies outta there!! I actually have several more jars...they are from the same mother flasks, but were replated a little later. MEANwhile....the next wave is doing well...I have some little Epi's coming up........Here's a pic!!
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  #90 (permalink)  
Old 05-07-2009, 06:17 PM
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Fantastic! I would wait a tad longer. Just to be sure they have a good start. You could do one flask at a time, and see how it goes each time.
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Old 05-07-2009, 07:21 PM
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Wow, you are doing great. I am so impressed with your first try at flasking. I hope when I finally get the chance to try this that I have the same good results you are having. Thanks for the update.
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Old 05-07-2009, 08:00 PM
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I'm totally impressed. They are looking really good. You are an inspiration. I will be watching this all the way to BLOOMS!!
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  #93 (permalink)  
Old 05-07-2009, 09:17 PM
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Quote:
Originally Posted by doodlekitty View Post
Ok let me try this again....

Here are some pics of the "chids"!! I'm thinking its time to get them outta there........can I get some advice from the experts???
Personally, I'd leave them in a bit longer until you get see a bit more root growth.
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Old 05-08-2009, 01:21 AM
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WOW.. your little darlings sure do look like they are coming along nicely! awesome job!

in fact, i am now inspired (thank-you) to attempt the "long journey" myself.....of course first i have to try and get a pod to play with, but while i am working on that i should have time to read up on the rest of the process, LOL.

here are a couple of pairs (a named pair and a noid pair) i'm trying to get to pod- hopefully 1 of the 4 flowers will accept my request
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File Type: jpg may 2009-B.jpg (43.7 KB, 9 views)

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Old 05-08-2009, 07:27 AM
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Impressed isn't the word. "Gobsmacked" would be more like it. (excuse the Yorkshire please)
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  #96 (permalink)  
Old 05-08-2009, 08:38 AM
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Quote:
Originally Posted by kninum View Post
WOW.. your little darlings sure do look like they are coming along nicely! awesome job!

in fact, i am now inspired (thank-you) to attempt the "long journey" myself.....of course first i have to try and get a pod to play with, but while i am working on that i should have time to read up on the rest of the process, LOL.

here are a couple of pairs (a named pair and a noid pair) i'm trying to get to pod- hopefully 1 of the 4 flowers will accept my request
Kninum, I think you and Exasperatus should hook up and exchange some pollen..
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Old 05-29-2009, 01:05 PM
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You are doing great. Personally, I would leave them in another month or two in order to get a good set of roots on them.

And I like the sounds of some of you guys... orchid sex always gets me going.
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Old 05-29-2009, 06:01 PM
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Doodlekitty, what method did you use to get the seeds into the sterile bottles in the first place???? I've read all the posts and just can't find the answer.

Bill
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Old 05-29-2009, 08:49 PM
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Quote:
Originally Posted by OrchidMom View Post
You are doing great. Personally, I would leave them in another month or two in order to get a good set of roots on them.

And I like the sounds of some of you guys... orchid sex always gets me going.
Thanks....coming from an expert, that's quite a compliment!
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Old 05-29-2009, 08:52 PM
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Gobsmacked!!

Quote:
Originally Posted by tizzycat View Post
Impressed isn't the word. "Gobsmacked" would be more like it. (excuse the Yorkshire please)
Ya know.............had I not been watching the Susan Boyle show (lol) I would never have known what that meant (Gobsmacked). What a great word!!!

And THANK YOU for the compliment!
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Old 05-29-2009, 09:01 PM
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Quote:
Originally Posted by willowbanks View Post
Doodlekitty, what method did you use to get the seeds into the sterile bottles in the first place???? I've read all the posts and just can't find the answer.

Bill

Oh man, it wasn't easy....

Sorry...not the answer you wanted. Short answer: Glovebox, baby jars and lots of bleach. Long answer: I guess I better get it all documented........I keep getting asked that question.

Seriously though, I performed a combination of many methods that I had been researching. Much like my approach to cooking, I threw together a bunch of "recipes" and came up with one of my own (method, not recipe). Trust me, I'm not being "coy"...just lazy I guess...as I never documented my steps (other than in my handy dandy laboratory notebook).

However I will be more than happy to answer any questions...and will certainly start working on documenting the process I have used so far...........
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  #102 (permalink)  
Old 05-29-2009, 09:14 PM
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Thanks Doodlekitty, I just had to ask that question, it's something other Geeks need to know before they get carried away thinking they can just open the bottle lid and drop the seeds in.

I've seen plenty of these sleeve boxes/glove boxes over the years but never tried one myself. I'm really impressed with the lack of contamination you have achieved, well done.

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Old 05-29-2009, 09:29 PM
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Thank you!!! You are really nice to say so! I was so worried about contamination I really probably went over-board with the bleach AND alcohol, for that matter. Anyway, you have motivated me to get my process documented (with pics) and since I have a couple of pods about to "pop" I'm getting ready for the next "go round" so I'll definitely be posting some helpful info soon.

I think the main reason I have been hesitant to post "instructions" is that I am a newbie myself and frankly, I really didn't think I'd have the success I had. So THEN I had to try it again and much to my surprise, I did it again!!!
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Old 05-29-2009, 09:36 PM
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Doodlekitty - I posted a question in another thread about the kitchen culture kits but I think it got buried in the numerous posts.

If I remember correctly there was only one kit to purchase on that site. Did you find that you needed additional items or was the kit complete other than the pressure cooker?

I am looking to get the same kit and have had it bookmarked for some time. I am waiting until I get a successful pollination of a new cross. Hopefully I will have as much success as you.
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Old 05-29-2009, 09:45 PM
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Hi Jay,

Yes you did and I saw it and yes it did.

I don't want to give you misinformation so I have to look up my records (I ordered it last summer) as to which kit...I also ordered the kit from Phytotech (epiphytes) and have used both. I will get that info for you tomorrow.

Now if I could be as successful at landing a job as I have been at propagating orchids, I would be a very happy girl. (Perhaps that is that answer to so many questions....I have plenty of TIME on my hands and spend a LOT of it on educating myself on orchid propagation).
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Old 05-29-2009, 11:00 PM
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Dear Rod,

Congratulations and reading your progress
with your flasks.

Thanks for sharing and also to RODCUDA for
his personal media formulation.
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Old 06-02-2009, 05:32 PM
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Kitchen Culture Kit

Quote:
Originally Posted by jay View Post
Doodlekitty - I posted a question in another thread about the kitchen culture kits but I think it got buried in the numerous posts.

If I remember correctly there was only one kit to purchase on that site. Did you find that you needed additional items or was the kit complete other than the pressure cooker?

I am looking to get the same kit and have had it bookmarked for some time. I am waiting until I get a successful pollination of a new cross. Hopefully I will have as much success as you.
Jay, The kit I purchased is the Basic Kit with manual, supplies and chemicals...it was $99.00. Not sure if the prices are still the same. Yes it was a great kit to get started with...and the founder, Carol has been great in answering follow-up questions, etc. I already had my own pressure cooker. The other "must have" is Aaron J. Hicks Book on Asymbiotic Technique of Orchid Seed Germination. I've ordered from his site as well....again....EXCELLENT customer support. Orchid people are just so nice!!
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  #108 (permalink)  
Old 06-06-2009, 09:39 PM
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How many flasks or how much of medium the kit produces? What is also included?
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Old 06-12-2009, 10:37 AM
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Quote:
Originally Posted by doodlekitty View Post
Well I must have done something right..........look what I have!!!! I'm not sure that they should be in little "globs" but I'm pretty sure I have germination!!!!!!
looks great, what medium did you use and how long did you wait after pollination until sowing


I just purchased knudons medium and some replate medium been researching like crazy...

Thanks just curious on the steps you took
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Old 06-12-2009, 01:00 PM
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OMG! this is soooo cool! I just came across this thread today and it's better than watching tv!

Doodlekitty, I've never grown a seedling, but if you want to pass them around, I'd be willing to "babysit" one for a few years and we can see which of us can get the first blooms! Have you named your Hybrid yet?
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Old 06-13-2009, 10:14 AM
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Hi vcuchick, thanks! You know what? I now have so many I am more than happy to send them to anyone who wants them. They are NOIDS (I have pics of the parents) so they're not worth anything...but where they lack in pedigree...they make up in numbers.....lots of em!!!

The tough part comes as soon as I deflask them (next month or so) because seedlings are HARD to raise. BABYSITTING is exactly what I would call it....
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Old 06-13-2009, 01:35 PM
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Ha! I'm not an orchid snob...half my collection are NOIDS, but they bring me just as much enjoyment as the ones with pedegrees!
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Old 06-29-2009, 12:04 AM
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Quote:
Originally Posted by doodlekitty View Post
Well I must have done something right..........look what I have!!!! I'm not sure that they should be in little "globs" but I'm pretty sure I have germination!!!!!!

Sniff Sniff... this is what my babies look like right now at the flaskers. Do you have any updated baby pictures?
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Old 06-29-2009, 09:31 AM
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Deflasked!!

Sure!! Last week I decided to take a leap of faith and deflasked 2 jars!! Here they are!!!!
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Old 06-29-2009, 10:09 AM
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they look great! good job!
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Old 06-29-2009, 10:42 AM
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Fantastic! They look really great!
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Old 06-29-2009, 03:24 PM
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Those look great- very green and healthy looking!

Makes me want to jump right in and flask something!

Wonderful job!
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Old 06-29-2009, 05:08 PM
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Thank you everyone!! I should post more often; you all make me feel so successful!!!
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Old 06-30-2009, 08:49 AM
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Wow, what a healthy looking bunch of plants! Great job Doodlekitty!!
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Old 06-30-2009, 11:19 AM
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So Cool! I dunno why I had been following this thread already lol! You are doing a great job on those! Yay!
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Old 06-30-2009, 09:23 PM
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So I guess its safe to deflask the other TWELVE jars?????

(After all, I have to make room for the 2nd batch of germinating babies...)
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Old 06-30-2009, 11:47 PM
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Go for it and good luck. 12 jars!!! You've got your work cut out for you. Have fun!
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Old 07-01-2009, 08:29 AM
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12 jars? Holy Smokes! Hope you have room for them! I'll take a baby off your hands when you run out of room!

I don't know if you've posted this already or if anyone has asked, but have you posted pictures of the seedlings' parent plants? I'm curious to know what they come from.
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Old 07-01-2009, 01:29 PM
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This should keep you busy. Where in the world are you going to keep all of these?
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Old 07-20-2009, 05:07 PM
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flasking

hi

not sure but I think you helped me in the past with a PDF document of how to flask and supplies your company has to offer
sorry if not you



Quote:
Originally Posted by doodlekitty View Post
Hi all....

I just completed my 1st flasking attempt. I've researched for months and months and THINK I did it all right but what I didn't research was exactly how to store the flasks. I flasked on Saturday and know I need to set them up under some lights but from what I read, they don't need much (they're dends). Help and thanks!
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Old 07-25-2009, 09:42 AM
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Yes it's keeping me busy alright.......LOL I just deflasked two more! I'm not planning on keeping them, but for now they've taken up residence in my spare bedroom. It's slowly turning into an orchid nursery...





Quote:
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This should keep you busy. Where in the world are you going to keep all of these?
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Old 07-25-2009, 10:48 AM
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Sure, I'm trying to post the pic.......apparently I am better at propagating orchids than creating jpeg files.....


Quote:
Originally Posted by vcuchick View Post
12 jars? Holy Smokes! Hope you have room for them! I'll take a baby off your hands when you run out of room!

I don't know if you've posted this already or if anyone has asked, but have you posted pictures of the seedlings' parent plants? I'm curious to know what they come from.
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Old 10-08-2009, 10:03 AM
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Its nice watching your babies grow!

I just recieved my flask, just doing a little more research before i take them out.

Just wondering, when your took your babies out of the flask, did you treat them in anything? I was told to use warm boiled water to clean the alger off the plants, then put in the compost and water with the fungicide provided. But it doesnt tell me to put into a plastic box to keep in moist and away from light for a few weeks.
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  #129 (permalink)  
Old 10-08-2009, 02:47 PM
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Thanks, I must admit they have definitely consumed a lot of my time. I have used several methods for deflasking and still feel clueless about the best way. I've tried the whole "rinse and separate" method, "leave 'em the agar" method and don't separate, use physan, don't use physan....sphagnum vs seedling mix, etc. I've definitely lost a lot of plantlets and some are still in flasks. I think the biggest issue is maintaining the perfect environment for them. For instance today it is a whopping 97 degrees in S. Florida and I had everyone outside, but had to bring them in. I have to defer to the experts on this one because I just can't seem to get it right.
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Old 10-14-2009, 09:17 PM
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Hey doodle,

How're the babies coming? picture updates??? Still willing to send me some babies?

Last edited by vcuchick; 10-15-2009 at 05:39 PM.
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  #131 (permalink)  
Old 10-14-2009, 11:32 PM
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This has been a great thread so far...looking forward to the updates.
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