
08-26-2008, 01:04 AM
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 | Junior Member  | | Join Date: Sep 2007 Location: Lexington, Kentucky
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Quote:
Originally Posted by markclkk I thought you have to inoculate with specific ectomycorrhizal fungus to germinate orchid seeds? | In the "wild" most orchid seed requires a mycorrhizal infection to germinate. In most cases, when a dry orchid seed comes into contact with water it swells and ruptures the seed coat. The seed will produce a few root hairs which the mycorrhizal hyphae will infect and continue up into the cells of the embryo, completely encircling the cell into what is called a peloton. This ball of fungal cells have a life span of a few days where after it consumed by the orchid cells and the embryo grows until the nutrient source is depleted. The cells will again become infected and the cycle will continue until the orchid is mature enough to produce chlorophyll where it will begin to produce some of its needed energy, while still keeping its mycorrhizal roots and consuming the fungus. Some orchids grow out of their dependence on mycorrhizal fungus, and some depend on it as adults.
With flasking today, all we need to do is supply the nutrients required for germination and growth. The sowing agar will have the nutritional requirements so there is no need for mycorrhizal cycle to provide sustenance.
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