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07-23-2011, 01:58 PM
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| My first flasking project just started.....
Well, wish me luck.....  I just started soaking Psyc seeds in the sweet water with soap.......  Seeds started dropping down to the plunger after adgitation.... There are gazillion of them there..... Oh boy!!!!! I think I added too much soap.....I'll wait till most of them drop and rinse them a little, I guess..... After getting ready for about one month - my jars with steril medium, syringes, caps, tweezers, bleach and of course my fish tank glove box are ready....So, I'll flask tonight or tomorrow morning......I started with my Psyc seeds because I have more flowers and always can pollinate more of them in case things go wrong. They are very easy to pollinate..... I'll let you know how it goes......  Last edited by orchidea; 07-23-2011 at 02:27 PM.  |
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1joyceh (08-14-2011), berkeleysgr8 (07-23-2011), Filb (07-23-2011), Greybeard (07-24-2011), orchids4me (07-23-2011), plantloverlisa (07-23-2011), Ron (11-22-2011), Shannara (07-23-2011), Susie11 (05-01-2012), tangers40 (07-24-2011), VEEKTOR (07-23-2011) | ||
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07-23-2011, 02:50 PM
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So tiny!!! I've always wondered about this process. So bizarre and fascinating  |
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07-23-2011, 02:55 PM
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Wow! Cool! Good Luck!
__________________ "DON'T YOU DIE ON ME MIA!!!" |
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orchidea (07-23-2011) | ||
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07-23-2011, 04:06 PM
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I just rinsed poor things from too much soap. I used Dr. Bronner's Magic Soap, so I hope I didn't kill the seeds....  And now they are just in the sweet water with a little bit of soap that's left there..... They look like they are ready, all of them are at the bottom.....the whole entire almost 1cc of them.... I wonder how many 4oz baby food jars should I sow these seeds to, I guess I'll start with 4 mother flasks....  I'll wait till it cools down a little so I can turn the AC off, so there is less air movement in the house and start flasking.......  Last edited by orchidea; 07-23-2011 at 04:12 PM. |
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07-23-2011, 04:09 PM
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I can't wait to do this some day!!! I'm sure your experience will come in handy when I do!
__________________ "DON'T YOU DIE ON ME MIA!!!" |
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orchidea (07-23-2011) | ||
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07-23-2011, 04:56 PM
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One thing I can share already.... DONT'T USE metal baby food jar's caps/lids for storing steril medium.... !!!I didn't have my newly ordered Magenta-B caps yet, so I used the metal ones that jars came with. They are getting badly rusted with bleach sprayed all over them....  I just autoclaved my Magenta-B caps, and planning to change them to these lovely plastic caps when I enter my "glove box" with seeds tonight. I guess, the best use of these metal original baby food jar's lids/caps is for replating. Then, they could be converted into the vented ones, and should serve nicely, as they don't need to be sprayed with bleach..... I am learning as I go.... |
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07-23-2011, 05:06 PM
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Looks good orchidea!! I didn't lknow anything about the step youjust did "sweet water" ? I know the 4 part water/1 pt bleach to sterilize every thing and the 1pt bleach /9 pt water to sterilize the seed and get the sinky seed as you did... Did I miss something lol  glad you posted this so soap what's the sweet part? ???Thanks Emmaye
__________________ Life is too short.... Buy more orchids!!!!   Emmaye |
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orchidea (07-23-2011) | ||
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07-23-2011, 05:21 PM
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Actually, look it up at OSP site. Aaron has a good step-by-step of what the heck to do..... . I am glad I posted this, so check it out. I know Frank didn't do it in his video, but later on in one of the posts, he suggested to do that......So, it's up to you.......
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orchids4me (07-24-2011) | ||
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07-23-2011, 06:30 PM
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Interesting proccess, you are much braver than I to do this. So Please keep posting your steps so we can follow along and maybe learn a thing or two!
__________________  Life is Good Today! Dream as if you will live forever. Live as if you will die tomorrow.  Synda |
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orchidea (07-23-2011), orchids4me (07-24-2011) | ||
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07-23-2011, 06:46 PM
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I am still waiting on my media to get here, and I wont be too far behind you, orchidea! I have one seed pod already dried out, and 4 more waiting to finish up. I hope they hold out until after I get the media.
__________________ Just one more orchid couldn't hurt, could it? ~~Cyndi~~ |
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07-23-2011, 06:51 PM
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oohhh very interesting thread, keep posting so we know how it is going! thanks!
__________________ HUG YOUR LOVED ONES DAILY |
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orchidea (07-23-2011) | ||
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07-23-2011, 07:23 PM
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So cool. My 16 year old son has been growing mushrooms from spores (no, not those kind of mushrooms He's growing pizza mushrooms (the name escapes me right now). It's a very interesting process. I think he's going to attempt to experiment with my orchids next.
__________________ My orchids.http://www.orchidgeeks.com/forum/members/retroresale-albums-my-orchids.html |
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orchidea (07-23-2011) | ||
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07-23-2011, 07:24 PM
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. That's what I did.I placed them into the printing paper, folded tightly. Then I placed some rice into the Mason jar (for the moisture control). I placed some minute-made rice..... Then I put the paper with seeds inside the Mason jar and closed tight....In the fridge they went..... One thing to keep in mind - fold the paper veeeeeeeeeery carefully. Seeds are very small, they go all over the place if not watched and folded carefully..... |
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07-23-2011, 07:32 PM
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Wow! That looks like a LOT of seeds! Good luck with this project... quite ambitious and very interesting. My inner science geek loves this kind of stuff.
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orchidea (07-23-2011) | ||
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07-24-2011, 12:45 AM
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I have to tell you guys....I feel like I went to Mars and back..... ...So, good or bad, here it is......  I couldn't make any pictures, sorry. Having my hands inside the glove box and wearing gloves it simply would be impossible......  I was clumsy, and two jars got very little seeds, and two others got most of them. Seeds wend all over the walls of one jar.... So, we will see what happens....I am not expecting much....but it's just a very interesting thing to do.... Even if I get contaminated flasks, I'll just do it again.....I think I will be much better at this next time...... |
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07-24-2011, 01:13 AM
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who did you order your media through, and which one did you use?
__________________ Just one more orchid couldn't hurt, could it? ~~Cyndi~~ |
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07-24-2011, 02:12 AM
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I ordered from OSP. I just told Aaron I need to flask Psychopsis and he shipped P669 which is a modified P668....I hope it helps. You actually can brew your own medium at home. I found many different recipies on line. The only problem is to know if it works for your seeds. There are some that claimed to be generic.... I think I'll try to brew my own next time. I am just waiting to get Agar...... |
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07-24-2011, 04:32 AM
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Looking good!!! I'm hoping you don't get contamination... It would be awsome igf it goes well on the first try I'm hoping when I finish getting the last things I need to get started here (foreceps and pressure cooker )Good luck!! :fingers_crossed: Wonder how long for germination. Some can take months and months. Man you making me get itchin to start up.....sooo exciting !!! 
__________________ Life is too short.... Buy more orchids!!!! Emmaye |
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orchidea (07-24-2011) | ||
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07-24-2011, 04:38 AM
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Orchidea...I meant to ask you other than in outerspace lol how did it feel to do this ?? Were you nervous at all? Confident now or all along ? Honestly I'm a tad nervous but excited and hope I can defeat contamniation .....or just by pass it lol wishful thinking lol Now for the green algea looking stuff were on track
__________________ Life is too short.... Buy more orchids!!!! Emmaye |
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07-24-2011, 08:41 AM
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Looks good If you're going to nuke your media flasks in an autoclave you don't need to wash them down with bleach so using the metal lids should be fine. Of course you have the Polypropylene ones now so you may as well use them. BTW, put your flasks in a dark place until you start to see some green. After that you can transfer to a brighter spot. Never expose them to sunlight though or the protocorms will burn.
__________________ Dan |
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07-24-2011, 09:03 AM
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Just yesterday browsing through the OSP site I found an article by Lars Jensen there, where he tells how to sow seeds without the glove box. I think I've heard it before, but I don't think I've seen it in print. Also, I don't think you must have a pressure cooker.....You can do it in the microwave now as you are going to have plastic caps... ... |
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07-24-2011, 06:06 PM
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Hmmm... that's an interesting article. I just wonder what the pharmacist will think when I ask for a box of 17 guage syringe tips
__________________ Dan |
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orchidea (07-24-2011) | ||
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07-24-2011, 11:30 PM
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 That is actually a reason I am not doing my own crosses yet, I am afraid to create a Frankenstein.... so I am into "selfings" to stay on the safe side........ |
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07-25-2011, 03:28 AM
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You 2 are just 2 funny
__________________ Life is too short.... Buy more orchids!!!! Emmaye |
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08-13-2011, 03:29 PM
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| Something is there.....
I would like to know what you think....I have no idea how it should look like, so I took few pictures.... I think I was too clumsy, so the first picture is to show how many seeds ended up on the sides of the jar...  The 2nd and the 3rd picture is to show what happen when you put too many seeds in one jar (I think).....   The last one, I think, looks OK, but I am not sure there are enough seeds there.........  Out of about 30 jars of medium, only one had contamination. I think it's a great rate taking in consideration that I had medium spilled (couple of jars overturned) inside my pressure canner..... |
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orchids4me (08-13-2011) | ||
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08-13-2011, 11:51 PM
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Hummmm... I'm not exactly sure what I see here... But the 2nd pic is striking me oddly. I think I will give Frankco a ping to come take a look at this. See what he says is going on. I haven't see him around lately but I'm sure he'd be very interested in this thread.... Good luck orchidea
__________________ Life is too short.... Buy more orchids!!!! Emmaye |
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orchidea (08-14-2011) | ||
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08-14-2011, 12:08 AM
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It's hard to see, and hard to tell what is there doing it for the 1st time. That's why I just ordered 30X magnifying glass on EBay..... ...It will come from Hong Kong in about 2 to 3 weeks LOL. I will prbably be able to see for sure what's happening there by then even without one........But let me tell you, you can see the contamination without any magnifying glass at all... In a way, I am glad I had one jar contaminated, so I could see how this stuff looks like...... |
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08-14-2011, 09:14 AM
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I think they look OK. I found some species will form a very pale protocorm on germination and green colouring only comes in when they start to grow leaves. They haven't been in the flask long enough to tell for sure. You should know within about 3 weeks.
__________________ Dan |
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orchidea (08-14-2011) | ||
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08-14-2011, 09:16 AM
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| Pictures of seeds
Hi orchidea, Congrats  The seeds look great so far. From what I can see that's pretty much how they should look. No worries about the seeds on the side of the baby food jars. Most of those should start to grow too. After the turn green you can tap the jar very lightly to scooch them down close to the media. Or, you can just let them be because I think you will have plenty of orchids with what you have in the baby food jars. You'll be surprised at how many you end up with. From the looks of the syringe you'll easily have over a thousand orchids. Good job on the presoak with “sweet water”. You should be proud, one out of 30 jars on your first attempt! There are seasoned veterans who would be very happy with that ratio. Now let’s hope we see some green! They should look like this in month or two.  This is what happens when you put too many seeds in a baby food jar. Still, they can be replated by scooping them out and spreading them in different jars.  Good luck! |
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08-14-2011, 09:59 AM
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Thanks Frankco for poping in for orchidea she will be a proud orchid momma to (thousands) soon . Wow she will be happy about your comment too as its the first time. I just hope I'm as lucky when I do mine very soon here Good job Orchidea !!!! And thanks again Frankco! Don't be a stranger Emmaye
__________________ Life is too short.... Buy more orchids!!!! Emmaye |
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orchidea (08-14-2011) | ||
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08-14-2011, 10:28 AM
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Oh, thank you so much for pulling Frankco into my thread, Emmaye. And of course, thank you SO MUCH goes to Frankco for taking time to look at my first attempt at flasking. Thank you Dan for your encouraging words too. You guys are just so supportive.......  Assuming I will re-plate without contamination, THOUSANDS of orchids...  .........Woooooouuuuuuuuuuu! I don't think I need THAT many..... , and just to make sure I have few, I pollinated another Psyc flower...........and few other orchids of mine....... I was surprised to see something happening in the jars with the seeds I ordered on EBay that were collected in 2004 and in 2005. They were stubborn seeds that didn't want to sink, so I was sure nothing is going to germinate from them. But they are doing something too. They are Den species. That's just fun! |
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08-14-2011, 10:59 AM
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Morning Orchidea!! Sounds like may need to find additional sources for jars . I wouldn't worry to much about the # of babies. You can always find homes here on geeKs later if you need to. I'm glad you did a bunch of pollinating. My latest attempts also fell off their spikes I don't have that touch to pollinate or something.... I hope I'm as successful as you've been when I start my flasks planning on getting my pressure cooker this week (don't trust our microwave its doing funny stuff sometimes ocassionally not heating ). I'm so excited for you !!!! And happy I don't know what to do right now lol I'm glad Frankco chimed in also I sent him an email was hoping he had some time Keep us posted momma !Emmaye
__________________ Life is too short.... Buy more orchids!!!! Emmaye |
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08-14-2011, 12:43 PM
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I really want to try this sometime and this thread is a great encouragement. Good luck orchidea and emmaye and keep the updates coming.
__________________ Jonada don't sweat the small stuff and in the end it's all small stuff |
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orchidea (08-14-2011) | ||
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08-14-2011, 02:16 PM
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It's actually a BIG pain in you know where.... ...bleach all over the kitchen, my clothes are all stained from bleach, mess everywhere. I got tired of taking my pressure canner and the jars up and down the stairs in and out of my basement, so I just keep everything in my dining, right next to my kitchen...... My house doesn't look like a livable space anymore......... I am glad I live alone in a big house, so I don't have to feel guilty about it.....My daughter came to visit right after I finished flasking couple of weeks ago, and she was concerned..... ......... I am sure she is wondering if I am loosing my mind or something. I always have an excuse up my sleeves, I tell her that that's what happens with age....But, I do want to take a chance to thank you for 60cc syringes. Emmaye was so kind to share few with me. She is just an angel, isn't she.... |
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orchids4me (12-03-2011) | ||
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08-15-2011, 01:22 AM
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I can't wait to see the outcome of this project! Thank you to the "veterans" like Frankco and Dan (terra australis) for the advice you've given on this project. Maybe others will be inspired to try flasking too! I'm not up for the challenge... and I certainly don't have any more orchid growing space in my home (my boyfriend probably already thinks I'm a bit insane... thankfully he has grown a lot of orchids in the past too, so he doesn't judge too much).
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09-12-2011, 09:14 AM
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 Hello Orchidea, any new updates on the flashing project?
__________________ Adelia The only two things I HAVE to do is breathe and die, everthing else is a choice. I choose to buy orchids  |
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orchidea (09-12-2011) | ||
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09-12-2011, 02:15 PM
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Thanks for your interest.... It's not showing any new contamination...That's a good news, I think.... I did find 2 little green bubbles inside one of the jars with Psychopsis seeds in it. I checked this morning, and there were 3 or 4 little green bubbles there.. I might increase the light for the one jar that has green bubbles in it to see if it's going to speed things up a bit. It's a very lenghty project.... , sorry for not having more exciting news...It could be very difficult to make a picture of these green bubbles, as they are just so tiny. But I will post pictures when there is something more of the substance there.... |
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09-12-2011, 02:41 PM
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How exciting I'll keep my fibers crossed seeing you this has given me inspiration. Don't know if I'll do it this year, but maybe next. Looking forward to updates.
__________________ Adelia The only two things I HAVE to do is breathe and die, everthing else is a choice. I choose to buy orchids |
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orchidea (09-12-2011) | ||
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09-12-2011, 04:07 PM
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How exciting! I'm very interested to know what's in the media. Is it proprietary?
__________________ Rachel  |
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orchidea (09-12-2011) | ||
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09-12-2011, 08:14 PM
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....Nope, I can share....First time I decided to go with the pre-packed formula I got from OSP. Aaron has bunch of different formulas and they are very reasonably priced. But for the re-plating I decided to check if I can brew my own medium at home. You can find recipes on OSP site as well or just google them.....there are many there....and some are very easy.... agar, charcoal, superthrive, 20-20-20 fert, bananas, etc - that's what I have used. But my brew didn't come out right....I'll have to find out what I did wrong....it didn't solidify after autoclave...Maybe too long in the pressure canner.... It looked fine before I put it into autoclave...I'll try again when I have time..... |
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Filb (09-12-2011) | ||
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09-12-2011, 08:36 PM
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Oh how exciting!!!! I can't wait to start my first flasking project
__________________ ~Jenny    |
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orchidea (09-12-2011) | ||
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09-12-2011, 08:46 PM
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When do you think you are going to flask and what seeds, may I ask? |
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09-12-2011, 09:45 PM
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I also ordered media from OSP, Unicorn Vessels from the home tissue culture group.
__________________ ~Jenny |
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orchidea (09-12-2011) | ||
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09-25-2011, 01:20 AM
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| First Picture of some life there!
This is super exciting...I finally could make a picture!!! Well, it's really not a very pretty picture, but you can see (well, if you really try.... ...) some green bubbles I was telling you about.  I was shocked to find a lot of them in this jar today. And this is one of the jars I flasked last..... But it's really exciting for me because it's a flask of Den. harveyanum, the one I really wanted to see to produce something..... Last edited by orchidea; 09-25-2011 at 01:39 AM. |
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09-25-2011, 02:49 AM
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This looks great I know different species or hybrids have different germination rate so this beat the Psychopsis ....I'm still learning the time frames my self. This is very exciting News here Orchidea!!!. Congradulations!!!Emmaye
__________________ Life is too short.... Buy more orchids!!!! Emmaye |
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orchidea (09-25-2011) | ||
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09-25-2011, 06:28 AM
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Congratulations - well done! Brooke |
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orchidea (09-25-2011) | ||
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09-25-2011, 08:28 AM
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| This is all I needed
I have a NOID phal and a NOID cat that I pollinated and the pod is going to be ready with in a couple weeks. This is great motivation and information to get me off my butt and flask! Thanks so much for posting all these pictures and information. What is the site you said to check out? Thanks, Johnny B
__________________ -Johnny B Flowers hold infinite beauty! |
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09-25-2011, 10:02 AM
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I know many flasking labs wouldn't flask tagged hybrids even, only species. After I flasked my Psych, all other ones were species Dens. Well, but if you want to flask them anyway.... ....and somehow I think you do........the website is on youtube, just type "flasking orchids" and Franks 3 part vedio is hopefully still there..... Good luck!
Last edited by orchidea; 09-25-2011 at 03:17 PM. |
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09-25-2011, 03:14 PM
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This is awesome! Congrats on the little green bubbles. I am looking forward to the progress and pictures! |
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orchidea (09-25-2011) | ||
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09-26-2011, 11:03 AM
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Good to see you are getting some results after all the effort I just bought a crate of bottles for further flasking projects. After your mention of using a hypodermic needle to sow in the flasks I thought I'd give it a go so I ordered some needles in a couple of guages to see what would work best. My thought is to drill a hole in the lid of the bottle (these particular bottles came with polypropylene lids) then fill it with silicone sealant to create a pliable skin-like membrane. I can then simply inject the seed through the silicone using a hypodermic needle and dispense with the need for a glove box or gallons of bleach. Thoughts?
__________________ Dan |
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orchidea (10-07-2011) | ||
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10-07-2011, 11:53 PM
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But I know from my other thread that you did it already. Good news, sounds like it works for you!!!! WOW, I might try this myself! What GA of needles do you think works best? You said you used 21GA...Don't you think the needle is too thin for seeds? I am thinking of ordering 20GA or thicker ones... What do you say? |
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10-08-2011, 04:07 AM
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I didn't have trouble with the guage but then most terrestrial seeds are generally quite small. What I did notice was that they tended to get trapped in the gap between the needle and the syringe tip. This was not because of size but more the design of the Leur press-fit attachment. A number of seeds just got trapped in the eddies within this space and it was impossible to get them out when injecting the flasks. I'll try 18GA next time as it may not have this issue due to the higher flow rate. 18GA is huge though. I was worried it would make a sizable hole in the silicone that wouldn't seal back up. How anyone would feel comfortable sticking one that size in a vein I will never know! More testing needed. Anyway, after all that I think it did actually take longer to do than using a glovebox (mainly from me trying to get the stuck seeds out of the gap). In summarry... Positive Don't need to construct a glove box Don't need to spray flasks down with bleach when sowing. Can re-inject easily at a later time if necessary (eg. you get interrupted in the process) Can accurately place seeds by aiming the needle - the silicone will flex. Negative Slightly more time consuming. Need to have suitable flasks. Risk of needle stick - injecting hydrogen peroxide into your bloodstream is a bad thing if I remember rightly.
__________________ Dan |
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11-22-2011, 08:13 AM
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Just bought myself a new toy Should work better than my microwave for sterilising flasks. 
__________________ Dan |
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orchidea (11-22-2011) | ||
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11-22-2011, 10:08 PM
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First time I worried so much my whole entire house is going to blust with it, my hands were shaking..... ....But nothing went ...Kkkkkaaaaboooom....and now I feel like a pro....BTW, the medium my little sprouting orchids are sitting in is drying up a lot, so I think you are right, there is air getting in/out somehow. So, good or bad, I am not going to stress over re-plating them. Thanks Dan for making me think in this direction, I was sure re-plating is the MUST.... Maybe my liquidy medium is not a bad thing to have afterall...it's drying up with some seeds there (I flasked just few jars to test), no signs of contamination....so time will show. Let us know how your autoclaving is going, please. |
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11-23-2011, 06:50 AM
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Will do. I was going to test it with a few flasks tonight to see how it would go.
__________________ Dan |
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11-30-2011, 09:48 AM
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Well... finally got around to pressure cooking some flasks. I love the capacity on this cooker - fits 7x 500mL bottles very comfortably. This batch came out at the perfect consistency. Very happy. Now to wait and see how well the cooker did its job.
__________________ Dan |
| The Following User Says Thank You to terra_australis For This Useful Post: | ||
orchidea (12-02-2011) | ||
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12-02-2011, 07:24 AM
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Cool... I know nothing about this things... Very useful thread..
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12-03-2011, 03:58 AM
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I'm hoping to get mine for xmas Orchidea: - any new up date pics ??
__________________ Life is too short.... Buy more orchids!!!! Emmaye |
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12-03-2011, 11:03 AM
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Hmmm... I'll have to post some of mine.
__________________ Dan |
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12-08-2011, 12:13 AM
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| The smallest orchids I have ever seen
This has been a very interesting project. It's just like magic, from such small seed I can FINALLY see a tincy orchid!!!...... I wish I could make a better picture, but at the base of each little orchid there is a whitish little beard...this is a clump of roots.....  |
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12-08-2011, 12:25 AM
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These look great!!! Was this jar the whole syringe of seed or part of a syringe ?? You got a nice number of babies in there, was this one of the dated seed pods you got from taiwain ? It also could be how they stored the seed over time/years. I look forward to seeing more of your flasking projects to come You did a great job!!!
__________________ Life is too short.... Buy more orchids!!!! Emmaye |
| The Following User Says Thank You to orchids4me For This Useful Post: | ||
orchidea (12-08-2011) | ||
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12-08-2011, 12:43 AM
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That's exactly what I was saying....I think, there were many more seeds there than the number of seedlings I have there right now. Not all seeds are germinating...... Dan had the same deal going. In a way it's kind of good, so maybe I wouldn't have to re-plate. That's a great thing if I could avoid re-plating - less chance for contamination. Dan was talking about it in another thread about flasking. These seeds are fairly new, not the old ones. |
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12-08-2011, 01:05 AM
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Orchidea - those babies are adorable! It's amazing how small they start.
__________________ Trying my hand at growing orchids since 2009. |
| The Following User Says Thank You to Emma Grey For This Useful Post: | ||
orchidea (12-08-2011) | ||
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12-08-2011, 01:16 AM
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My Den. speciosum babies are looking very similar. Getting some cute little leaves like that and a bit of root fuzz. speciosum is quite slow growing though so I expect yours will mature much quicker.
__________________ Dan |
| The Following User Says Thank You to terra_australis For This Useful Post: | ||
orchidea (12-08-2011) | ||
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12-08-2011, 03:38 AM
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Some of my results...   
__________________ Dan |
| The Following User Says Thank You to terra_australis For This Useful Post: | ||
orchidea (12-08-2011) | ||
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12-23-2011, 01:48 PM
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WOW! Looks like you are doing great!! I don't think I would feel safe doing it myself. LOL You are so brave to do this on your own.
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