Making your own medium?

[rls1989]

Does anybody have any recipies for the medium to use for growing the orchid seeds?

[Ellen]

The best recipe is to send the seeds off to a reputable lab and have the propagation done professionally.

[orchid_lord]

I am also very curious. At last, I pollinated a phal and I expect some seeds. I don't think there are this kind of labs in my country... There must be some ingredients or something, even if it is very complicated. But, with the will of God...

[Fabolusus]

Node culture in soil?

You could try this method. I don't think it works for all orchids, though

[orchid_lord]

Do you think it works on phals?

[Joseph Stanly]

Dear,
Old flower stems of phalenopsis can be induced to produce keikis or small plant-lets by swabing with kieki fix. ie a cream like lanolin which contains a chemical like BAP{6 Benzile Amino Purine ]. If you are aware of this material in the market , just scrub the old scape or small leaf like covering in the old flowered stem with a blade or scruber
with out damaging the eyelet in side . Apply the paste[keiki fix] lightly with a swab and wait for some days, the eyelet will swell and produce a small plant, and grow roots. After the plant has sufficient root length and one or two leaf, remove the plant with roots by a gentle twist and plant in spagnumn moss ion 50% shade in a humid place. Some advanced methods [like growing in a terrarrium]and culture media methods in side a steril flask are given in AOS Magizine. In future I will give you the date of publication if you are interested.
Thanks,
Joseph Stanly

[gerneveyn]

Here is one I found but have not tried.
Sugar: 20g/Lit
Fertilizer: 3gram 20-10-20 soluble, no urea (urea is not good for seed germination). According to some sources, this is almost the same MS formula.
Bannana: 100g/Lit
Coconut water: 100ml/Lit fresh from grocery store.
Vitamin: 1 tablet of centrum /Lit
Distillated water: add to make 1 Lit
Agar: 8-10g/Lit
pH: 5.2

[orchid_lord]

thanks gerneveyn. But how can I control the pH? Is it really important?

[gerneveyn]

pH is important and can be adjusted by adding NaOH or KOH or HCl as necessary. Here are some links that may be helpful:
www.phytoctechlab.com see technical literature and tissue culture techniques
http://web.telia.com/~u11206828/engindex.htm
http://instruct1.cit.cornell.edu/cou...d/orchid1.html
http://personalpages.bellsouth.net/f...ks/orchid.html
http://orchidseed.com/
http://www.orchideenvermehrung.at/english/index.htm
http://members.iinet.net.au/~barryg/CONTENTS.htm
http://www.kitchenculturekit.com/sivbposter.htm
Good luck. Aseptic technique is critical if you're going to grow the seeds on agar because contaminants grow faster.

[orchid_lord]

What's the aseptic technique? Isn't it something where you use sterilized tools?

[bogdan_orchidboy]

You have to use sterilized tools, but if you don't have a sterile workplace, you have done nothing.

First try to grow better your orchids and then I will teach you to grow aspetically.

Regards

[orchid_lord]

How do you know how I am growing my orchids??? PS: I know how to work aseptically .

[BlakeeBoo]

Is coconut water just the water from inside of a coconut

and could you use gelatin instead of agar

[BillC]

For all the subscribers to this thread. The following text is from Chula Orchids newsletter and may be of some interest.

Suggestion -

Now, let me explain something here tho to all the beginners out there. This is NOT the way to make a whole bunch of plants. Professional growers may use it to make a couple of duplicates of some fantastic plant they do not want to go to the expense now of merstemming or perhaps are in danger of losing. It's rarely fun, for a hobbyisst this process may well take over two years to get a keiki with roots that can be safely taken off the plant.

So if you an your friend are both going to use it on plants you purchased at Home Depot so you can "share", i suggest you forget it. You will be way better off to just go purchase another small plant or purchase phal seedling from some catalog. Don't waste you time on this Keiki process. It will be much more costly in time and effort than purchasing another fine seedling will ever be. You will have a two year head start on anything you may get using keiki paste. If anything you have receives an FCC from the American Orchid Society, by all means, use the keiki paste to reproduce that for your buddies, otherwise leave this keiki paste to the professionals.

Bill

[BlakeeBoo]

wow it sounds like you need to settle down the keiki method is very successful and if you think about it if you have one plant it successfully doubles your phal collection. Another thing just because people are new to this website does not meen they are new to orchids

[BlakeeBoo]

were did you get this recipe