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Old 04-18-2012, 10:35 AM
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Question Mericloning from seedling?

I was talking to acouple of nurseries at the Sydney Internation Orchid Fair about Mericloning some orchids I have. From my understanding I need to grow a meristem from backbulb of my mother plant and send/bring to the labs for the culture. But I was wondering, can mericloning be done on a seedling/mericlone that just came out of a flask?

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Old 04-18-2012, 10:44 AM
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All a lab needs is a "new growth" from a plant to extract the specific tissue to create a mericlone. The Lab I use requires a ng of about 2/3rds grown ( for a Catt )to work with.
Other plants have diferent requirements which also ary between labs.
A seedling from the flask can be done but is easier while the seed is still i the corm stage. Not sure why you would want to do this tho, expensive and pure chance on an unknown quantity.
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Old 04-18-2012, 11:33 AM
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yes, how much did they say it would cost? (just curious)
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Old 04-18-2012, 11:39 AM
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I had the same thought as nugget, why would you want to mericlone an un-flowered seedling? My experience with seed crosses is that many are not superior to the parents.
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Old 04-18-2012, 12:06 PM
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Generally meristem tissue is taken from either new pseudobulb or flower spike growth. The tissue is decontaminated and carved up into small pieces which are transferred to sterile nutrient solution. After X amount of time the tissue starts to grow protocorm-like bodies. From there you can multiply by further slicing and dicing these little growths, waiting for further PLBs to grow and so on.

I can't remember why but you can't mericlone plants that are mericlones themselves. I believe it was something to do with the genes degrading. It was explained to me as being like a photocopy of a photocopy of a photocopy...

To answer your question above, yes, you could mericlone a seedling from flask but the odds of it being a worthwhile investment are extremely small. Mericloning is usually reserved for extremely good examples of a particular cross in order to multiply the plant without waiting the time needed to propagate by division.
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Old 04-18-2012, 07:44 PM
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Quote:
I can't remember why but you can't mericlone plants that are mericlones themselves. I believe it was something to do with the genes degrading. It was explained to me as being like a photocopy of a photocopy of a photocopy..

You can but the risks of defects are increase each time you repeat the mericloning process.
Cost can vary form plant to plant and most do only a minimum of 200 plants
Cost can vary from plant to plant depending on the degree of difficulty, some from $2 a plant upwards
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From my understanding I need to grow a meristem from backbulb of my mother plant and send/bring to the labs for the culture.
As for Cymbidiums a new growth about 12 long leaves is often enough.
As for Phalaenopsis they will destroy the original plant to get a piece off it for cloning.
Best to contact a lab and ask.
Often it is best to chase up the nursery were you bought you original plant from and see if the can sell you a flask or extra young plants for example Cymbidiums seem to be no problem in chasing extra plants.
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Old 04-19-2012, 07:04 AM
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Ron they tissue culture a Phal using the spikes.

Brooke
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Old 04-19-2012, 08:41 AM
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I wanted to increase my collection of Chinese Cymbidiums. But at $150/flask, its really quite expensive. If I can get say 200 mericlones of a mericlone for half the price, i guess its worth wild. But if it increases the percentage of defects, then I might try to mericlone from a mother plant instead.

Ron, do you know any good labs around?
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Old 04-19-2012, 06:44 PM
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Quote:
Originally Posted by Brooke View Post
Ron they tissue culture a Phal using the spikes.

Brooke
Some do and some still use the method of taking it from the main growing center of the plant.
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Old 04-19-2012, 06:48 PM
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Both I believe do good work, I have bought flasks from both of them but have not used their mericloning service.

Home Page
David Keanelly Orchids | Cymbidium Orchids Australia

PAULINE’S LABORATORY SERVICE
FOR ALL ASPECT OF CYMBIDIUM
TISSUE CULTURE
CONTACT PAULINE OR KEV McLEAN
PHONE…(08) 8386 0430
POSTAL ADDRESS…13 WENTWORTH ST.
MOANA
S.A. 5169
EMAIL….kevin.mclean3@bigpond.com
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Last edited by Ron; 04-19-2012 at 06:53 PM.
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Old 04-20-2012, 10:20 AM
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"You can but the risks of defects are increase each time you repeat the mericloning process."

Ron is very correct here, most / all of the peloric cymbidiums for sale that have been known under different clonal names have been created by multiple mericloning. ie, mericloning a mericloned, mericloned, mericlone etc.
This process is not knew either. I catalogs of recent times, last 10 years, NEW mericlones have been advertised for sale of plants that were over mericloned 30 years ago, wonder what the heck someone will flower ( if ever )
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