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Old 08-09-2013, 09:25 PM
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i think it is my first home run

my first batch of commercial media flasking with P668. my first three attempts with homemade media only yielded protocorns, not growth after that and all was discarded.

initially saw some "activity" like swelling of the seed clumps, then they grew slightly bigger and today i can see greenish area. i think it has germinated! the pics are a little bit blurry with my digital camera, sorry for that. i dun think my slr can do a better job because it is the standard lens that came with it.

now the question is when is appropriate time to do re platting? many thanks
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Old 08-10-2013, 01:12 AM
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Hi difficult question to a degree, while I have not done flasking I buy a lot of flasks and have seen my nursery mate replate them.
Most plants grow rather quickly from seeds and you have a lot of seeds in your mother flask.
usually replateing is done when the plants are large enough to handle and be able to divide to individuals, say Cymbidiums for example, I have seen them replated around 1/2 to 1 inch in side and about 30 to a 4 inch wide glass jar.
Best keep the agar about 3/4 of an ich thick so the young plants can grow to a nice size in the flask before they need to be taken, remember sterilize every thing you use when replating.
Have fun and enjoy the new plants.
Here is how they do it on Utube
Replating Orchid Seedlings - YouTube
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Old 08-10-2013, 06:30 AM
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Hi difficult question to a degree, while I have not done flasking I buy a lot of flasks and have seen my nursery mate replate them.
Most plants grow rather quickly from seeds and you have a lot of seeds in your mother flask.
usually replateing is done when the plants are large enough to handle and be able to divide to individuals, say Cymbidiums for example, I have seen them replated around 1/2 to 1 inch in side and about 30 to a 4 inch wide glass jar.
Best keep the agar about 3/4 of an ich thick so the young plants can grow to a nice size in the flask before they need to be taken, remember sterilize every thing you use when replating.
Have fun and enjoy the new plants
thanks you soooooooo much for the video.

yup, i accidentally pressed too hard on the syringe plunger and delivered too much at one go. well, guess i got to move my bed room to the store room soon, i am running out of space for orchids!
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Old 08-10-2013, 06:56 AM
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So exciting Ian!!
Congratulations and keep us posted
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Old 08-11-2013, 09:50 AM
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Quote:
Originally Posted by catasetum-ian View Post
my first batch of commercial media flasking with P668. my first three attempts with homemade media only yielded protocorns, not growth after that and all was discarded.

initially saw some "activity" like swelling of the seed clumps, then they grew slightly bigger and today i can see greenish area. i think it has germinated! the pics are a little bit blurry with my digital camera, sorry for that. i dun think my slr can do a better job because it is the standard lens that came with it.

now the question is when is appropriate time to do re platting? many thanks
so on your first three attempt did you not replate?? did you use the same media for germ as well as replate??
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Old 08-11-2013, 11:31 AM
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so on your first three attempt did you not replate?? did you use the same media for germ as well as replate??
first three attempt only yielded some yellow protocorn, did not turn green. i took a couple of shots and ask some senior growers, they said "does not looks like it". so did not attempt the replatting and throw the flask away, the longest i kept was 2 years.
this one is different, the protocorn turns green they next day they germinated.
so when should i replate this one because i put in too many seed in this one.
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Old 08-12-2013, 09:36 AM
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I guess it depends on type..i am going to replate my phal seeds at 2-3 centimeters or 2 months,witch ever comes first
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Old 08-12-2013, 10:13 AM
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I guess it depends on type..i am going to replate my phal seeds at 2-3 centimeters or 2 months,witch ever comes first
i just found some info from the net, it says that replating can be done as early as once the protocorn is large enough to be manually handle. the only setback is the larger the protocorn is allow to to develop before replate, the higher the survival rate. the only setback for me is i squirt too much seeds in this one, so i guess i do not have the luxury of waiting here.
well, on the other hand if i am lucky, i will end up with more flask. hehe

just an update of them today
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Old 08-12-2013, 10:34 AM
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Quote:
Originally Posted by 2woodensticks View Post
I guess it depends on type..i am going to replate my phal seeds at 2-3 centimeters or 2 months,witch ever comes first

We usually leave the protocorms for about a year before replating. I have even had some get big enough in the original flasking jar I could compot them up and skip the replating. Right now I have a flask of Phal. aphrodite that needs to come out and potted up.

Remember, the more times you replate, the more chance of contamination.

Brooke
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Old 08-12-2013, 10:47 AM
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We usually leave the protocorms for about a year before replating. I have even had some get big enough in the original flasking jar I could compot them up and skip the replating. Right now I have a flask of Phal. aphrodite that needs to come out and potted up.

Remember, the more times you replate, the more chance of contamination.

Brooke
yes very true Brooke, the more replate the more chance of contamination. but i am just afraid they would be overcrowded and eventually die of undernourishment.

and wow! skip replatting? first time hearing this because i was told that replating medium is different from sowing medium because they had banana powder in it. i think i must try to sow minimal amount of seeds in a jar the next time to experiment of this, the skipping of replating, many thanks
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Old 09-05-2013, 11:23 AM
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replatting 。。。。。。

well, here it goes. my first replatting. but at the mid of doing it, my back is killing me and i was asking why am i doing this and also i will be thinking no more flasking for me!
but looking back (the second and the third photo) on the final result.........hehe...i think i might do it again!!! haha

i took the advice given here, fewer plants in a flask so less replatting, in fact i hope they will last till deflasking is ready

but i was so tired in the mid of it that i think i broke sterility....keeping my finger cross here
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Old 09-06-2013, 06:08 AM
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So exciting Ian!!
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Old 09-06-2013, 07:27 AM
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Good Job!!!!!Flasking has always intrigued me.
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Old 09-06-2013, 08:29 AM
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Congratulations...this is so exciting. I don't know the first thing about flasking, but it looks like you are doing an excellent job.
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Old 09-06-2013, 12:08 PM
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Cute little green spots! It's going to be fun to follow their growth via your reports, so keep them coming, Ian.
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Old 09-06-2013, 05:26 PM
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Good luck and thanks for posting. Who would think that the little smudges of green in your first photos are destined to be leafy blooming plants in a few years. It's a very interesting process.
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Old 09-06-2013, 06:58 PM
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Cute little green spots! It's going to be fun to follow their growth via your reports, so keep them coming, Ian.
Yes, yes. Keep the updates coming please.
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Old 09-11-2013, 04:05 AM
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This is fantastic Ian very exciting!! Keep us posted!!
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Old 09-11-2013, 06:07 AM
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I find the best time to replate is when the protocorns start to develop roots. Up until then, they don't need it.
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Old 09-11-2013, 07:39 AM
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I find the best time to replate is when the protocorns start to develop roots. Up until then, they don't need it.
ooopsss.....i guess a little too late, well sort of.
coz i had already replated them because i had squirt in too much seeds, they ended up as a green layer or carpet and basically no space between them. i read in the net that too much plant together will build up the toxic phenolic waste, and that is why i replated them. the only roots i can see are those tiny root like structure (sort of like small spike)

secondly i said "sort of" because i still had abundance of them in the original mother flask left.
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Old 09-12-2013, 06:33 AM
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Shouldn't matter, just the later replating eases the mortality and you pick the biggest strongest ones from the pack.

Keep the mother flask and you can assess down the track and do it all again.
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Old 10-03-2013, 07:58 PM
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here are some updates on those replate flask that are not contaminated. i can see shoots are growing, no roots yet. the pics are blur with my digital camera, hard to get it to focus with the glass interface.
(details on my flasking attempt is posted in my blog recently, would like to hear any further comments about my materials and methods, looking for space for improvement)

many thanks
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Old 10-04-2013, 06:47 AM
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How many of the replate flasks did you lose?

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Old 10-04-2013, 07:05 AM
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How many of the replate flasks did you lose?

Brooke
well, about three quarter of it, i knew i shouldn't have taken my hands out of the sterile chamber but my back is killing me after a couple of minutes bending down. well, got to improvise on my fish tank, should start doing one with rubber gloves and silicone sealing
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Old 10-06-2013, 06:48 AM
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Sorry for you losses but it looks like you will have plenty survive.

Brooke
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Old 10-25-2013, 10:15 AM
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How are your replate flasks doing, what size are the plants now?
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Old 10-25-2013, 06:58 PM
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How are your replate flasks doing, what size are the plants now?
as expected most of the replate flask are contaminated after i took my hands our of the sterile chamber and break sterility but three survived so far.
the protocorns are about 1cm plus minus, the largest one with two small leaves, like those new keiki when they start emerging from a PB with a "V" shape. some protocons are with one roots, some is still without roots.

i notice that the protocorns that are replatted at much smaller size in the beginning develop massive protocorns multiplication before sending out new shoots.
those protocorns that are left to grow bigger before replate send out shoots faster but less multiplication noted

will post up some pics if they made it to a plantlet size
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