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Old 12-01-2008, 09:47 AM
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Finally flasked...now what?????

Hi all....

I just completed my 1st flasking attempt. I've researched for months and months and THINK I did it all right but what I didn't research was exactly how to store the flasks. I flasked on Saturday and know I need to set them up under some lights but from what I read, they don't need much (they're dends). Help and thanks!
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Old 12-01-2008, 11:04 AM
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wait for colour change. hopefully they change from white to yellowish and not black.
then wait for the protocorn then you tranplant the protocorn, then you wait for it to grow
it true leave, then you wait for the root, the you wait, ... then you wait.... then you wait... see your seedling in 1 year time.
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Old 12-01-2008, 11:50 AM
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Thanks digital..........yes 10 years of collecting orchids has prepared me well for WAITING!!! What about the light requirements?
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Old 12-01-2008, 09:34 PM
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1000 foot candle about 3 inch from the FL tube. any FL tube should be fine. Also depend on the type of orchid you are growing. 1000 seem a lot. by the time the protocorn emerged
it will drop to 800foot candle , so dont worry about it. also don't put all your egg in one basket. having two flask of the same thing help to minimize the loss.
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Old 12-01-2008, 11:22 PM
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Thanks for the info.........I flasked Dends (3 jars each). I also flasked some Oeceoclades maculata....they're practically growing like weeds in S. Fl....so I thought I'd practice with a few pods.
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Old 12-25-2008, 05:56 PM
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Well I must have done something right..........look what I have!!!! I'm not sure that they should be in little "globs" but I'm pretty sure I have germination!!!!!!
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Old 12-25-2008, 09:13 PM
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Houston, we have lift off!! It looks good! Someone with more experience will give you a better time frame, but I know that after they get large enough, you'll plant them on in new flasks, dividing the "blobs". Congratulations!
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Old 12-25-2008, 09:50 PM
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Hi, they are germinating well, looks good.
My mate who has a flasking buisness say to wait untill they are a size you can handle! then replate into another flasks.
Others will offer advice as well, good to see some have a go at it.
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Old 12-25-2008, 10:17 PM
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Thanks Doodlekitty. Great try. It is the first time Im seeing them at this stage.Please keep posting us.
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Old 12-25-2008, 11:14 PM
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thank you...I can't believe it!!

I was too scared to actually SAY that they germinated...but after spending too much time with a flashlight and magnifying glass....coupled with a new camera, I just had to post!!! Wow I'm just amazed that I got it on the first try....I don't want to get too excited though....i know I have a long way to go!!
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Old 12-26-2008, 07:07 AM
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OOOOOooooo this is fun
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Old 12-27-2008, 10:03 AM
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This is fun! I'm just hoping I can continue to be successful! Any ideas on how long it will take for them to become protocorms???
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Old 12-27-2008, 12:54 PM
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Great job doolekitty!

I think the stage they are in now is protocorm. Is that right? You just have to wait for them to get a little bigger so you can separate individuals.
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Old 01-03-2009, 12:13 PM
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Hi all and thank you so much for the encouragement!! Well the (I guess its safe to call them "protocorm") are getting bigger!! Now I'm concerned about the lighting requirements......AND when I'm supposed to replate. There is a lot of mixed views on the subject........anyone have a suggestion???
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Old 01-03-2009, 12:40 PM
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facinating stuff, keep it coming.
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Old 01-03-2009, 01:32 PM
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You have a ways to go before you need to re-plate. I would use grow- lights, since you can regulate the timing, and not worry about burning the plants with direct sunlight. There are others that know better, but that's what I would do. I have some seedlings that I'm growing under lights, and they seem happy!
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Old 01-03-2009, 02:31 PM
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Thanks Patticake...I'm probably "jumpin the gun" worrying about replating.....sheeesh....I did'nt think I'd even get THIS far so soon. The more you read the more confusing it gets about the timing of the replating. Some say to do it before root formation, some say wait until after......they seem soooo tiny but then again they're growing fast!
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Old 01-03-2009, 02:38 PM
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Doodlekitty - I have a Paph cross in pod that I duplicated several months ago. I planned on giving it away to someone that wanted to send it to a flasker, but if you are interested I could send it to you when it is ready. It is Paph Mamie Wilson (lowii x primulinum). I have the primulinum as the pod parent, which is opposite of the normal hybrid.

I planned on just giving them away to the forum to possibly use as another project plant since I do not have room for a whole new flask of seedlings.

Let me know if you are interested. I really don't know when the pod will be ready, it is still very green. I will try to figure out when it was pollinated, I was lazy and forgot to write down the exact date.
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Old 01-03-2009, 07:19 PM
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Absolutely!

I'd love to adopt your pod! (You may want to look for a more suitable parent, though!!)

Are you sure you don't want to give it to an expert flasker?
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Old 01-04-2009, 01:06 AM
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This would make it a true OrchidGeeks project. I really don't know when it will be ready and what to do with it. I will post a picture of it soon.
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Old 01-04-2009, 09:11 AM
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Great! And for the record...I MEANT to say in my previous post: "are you sure you DON'T want to give it to an expert flasker"???

I kinda "winged" my first flasking attempt (which is why I'm so amazed that I was successful) but I plan to fully document the next one, so I'll start with the pic you post.

Btw, my seeds were flasked green pod and I'm thinking I'd like to do the same with your pod...so from what I've been reading, its best to use the pod at about 2/3's maturation time...so a good guestimate as to when it was pollinated would be just fine.
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Old 01-04-2009, 11:30 AM
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Well, as accurate as I can be, I think it was pollinated around Sept 16th. I counted all of the previous blooms on the primulinum (10 so far) and divided it by the amount of time since I noted in my records when the first bloom happened. I then counted out where the pod was to get a guess.

Anyone know when I should harvest this pod to get it ready for flasking?
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Old 01-05-2009, 02:29 PM
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Jay,

For Paphiopedilum primulinum and its hybrids I would say harvest your pod at about 135-150 days. Provided you are not sure on your exact date I would recommend favoring the lesser time frame and possibly harvest at about 125 days to give yourself a buffer.

I've done some work with primulinum and my results felt very close to this window. Paph. primulinum and haynaldianum tend to have the shortest green pod times that I know of within the paph family. Most of the others tend to vary between 240 to 300 days from pollination based on my observations. With only one occurrence of Paph. purpuratum hybrid taking 361 days. Still can't really explain that one.

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Old 01-06-2009, 10:29 AM
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Holy Moley....are we looking at the mid-Feb time-frame? Thanks for the info Troy.....
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Old 01-06-2009, 04:23 PM
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That is crazy! Im just trying to keep the plants I have alive. You have great courage!
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Old 01-06-2009, 07:51 PM
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Great! Then I will count the days and harvest the pod in Feb. Doodlekitty, I will PM you with more information in the coming week. If you PM your address to me, I will send the pod out as soon as it seems ready. This could be fun for the whole forum!

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Old 01-07-2009, 12:18 PM
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Ok will do! You mean the WHOLE FORUM will be watching??

That's a LOTsa pressure!!!
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Old 01-07-2009, 02:01 PM
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Hi, congratulations, I flasked some phal seeds in november and they are just turning yellos, you must haave done something right!!!
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Old 01-07-2009, 06:22 PM
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Hi Doodlekitty. This is a great thread.
I tried to make my own flask few years ago but without success. And my problem was the agar mix. Maybe you can help me out now since you were successful with yours, how did you prepare the mix and what was the proportion that you use? Thank you.
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Old 01-11-2009, 05:11 PM
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Well here is the pod and the plant. I have had to wrap the spike around to keep it manageable. I will wait just a little longer before sending it to you.
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Old 01-11-2009, 05:23 PM
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Quote:
Originally Posted by doodlekitty View Post
Ok will do! You mean the WHOLE FORUM will be watching??

That's a LOTsa pressure!!!
Don't worry, we're all nice! I had some seed capsules that were no good in the end, but everyone was very nice. We can all learn from you, especially if it works!!!
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Old 01-11-2009, 08:25 PM
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Wow! I feel like I'm meeting the "birth mother"!!!! Thanks for the words of encouragement, Patticake!
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Old 01-13-2009, 11:31 PM
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Back to the original subject of this thread......

Check out the little babies.....they're growing horns!!!!!!
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Old 01-14-2009, 08:36 PM
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Very nice! I can't wait to send this pod to you to see if it is viable.
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Old 01-15-2009, 01:13 AM
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That is so cool and I'm am watching this thread like a hawk.
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Old 01-15-2009, 01:43 AM
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Wow! This is so cool!

I hope that Jay's pod will do as well. I watching too!
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Old 01-15-2009, 08:51 AM
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Thanks for your support....You guys DO realize that I'm a novice right??

I've been reading/researching for a long time now but the experts tell me the best way to learn is to JUST DO IT!! (So I did). I sure hope I have success with the "Orchidgeek Pod".
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Old 01-29-2009, 09:23 AM
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Hey Jay....

How is our pod looking????
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Old 01-29-2009, 07:25 PM
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It is still very green with the slightest of pleats. I think I will try to give it another week or two before sending it. I did get your PM with your address. I will let you know when I think it is ready. Not really sure, but will guess.
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Old 01-30-2009, 09:05 AM
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Ok no rush!! I hadn't posted in a while and just thought I'd check on the little tyke. I think I'm going to replate some of my babies...they look so crowded and are getting tiny roots. Since I have 5 jars, I thought I'd experiment with a couple of them.
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Old 01-30-2009, 10:00 AM
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hi doodle kitty please post a pic of last 15 days' developments.... Thanks for this interesting thread ...
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Old 01-31-2009, 12:25 PM
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You're welcome! Ok here are some pics of the latest.
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Last edited by doodlekitty; 01-31-2009 at 12:28 PM. Reason: add pic
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Old 01-31-2009, 01:05 PM
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Awesome!!! Keep it up! They look fantastic!
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Old 01-31-2009, 02:52 PM
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Hi everyone.

I have been following this thread with major interest for quite some time now. I like the way things are progressing with doodlekitty's seeds.

I had 4 odontoglossum seed pods, now 3 since 1 got broke off, on the same spike. I don't know what they were pollinated with. I am thinking of germinating one of the pods.

This plant came from Hoosier when they were selling out their plants. Would anyone be interested in one of the pods? For free, of course. I don't know what is involved with shipping etc, since I have never sent any orchid related items out.

Maybe doodlekitty would like to try? Its kinda risky I think not knowing what the plant is crossed with. But I figure, with it being Hoosier, it must be something really good.
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Old 01-31-2009, 05:51 PM
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Hi Doodlekitty.
Looks like they are coming along nicley, curious as why they "seem" to grow down the side and some inside the agar.
Is it semi solid like a good jelly or more softer.
The ones on the top of the agar look like they are forming leaves and coming along very nicely.
best of luck
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Old 02-01-2009, 10:23 AM
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Thanks Ron...yes I'm also concerned/curious about the ones in the agar..I'm not sure thats good....and for the most part all of the protocorm are on top. I used the same agar for these jars so not sure why it's consistency is a little softer....all part of the learning process, I guess.

Sarah, if you want me to give it a try, I will...but I must reiterate that I am a NOVICE!!! I'm not picky....I'll take a NOID any day!!!
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Old 02-01-2009, 05:42 PM
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Great, I will PM you and talk some details.
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Old 02-07-2009, 10:03 AM
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Ok, wish me luck guys......I'm going to reflask some of these guys today.......got the media ready to go over a week ago and so far looks good, no contamination.
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Old 02-07-2009, 10:18 AM
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Go, Doodlekitty! Good luck!!
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Old 02-07-2009, 12:16 PM
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Also some pics ....
As many pics of the process as possible please.... without of course hampering your actual process...
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Old 02-09-2009, 11:23 PM
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So here we are......the results of my first replate!! I have about a bazillion protocorm but only reflasked 1 jar: 4 jars worth. Like before, I'm convinced I botched the whole thing but I guess I'll have to WAIT (always waiting) and keep fingers crossed that I don't see any contamination. Here are the "chids".
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Old 02-10-2009, 03:23 AM
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The protocorms look good! I'm keeping my fingers crossed for you too!
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Old 02-12-2009, 01:40 PM
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i took advice and started reading on seed propagation. Boy that is tough and needs a lot of determination and care, especially keeping everything sterile. Your thread and various steps through the curent protocorns helped me to better understand the proccess. I am not sure i could make it. I have a self pollinated cymbidium that starts changing from white to green with the help of some bugs. I hope that by the time there will be a pod (if any) next year i could try myself.
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Old 02-12-2009, 06:26 PM
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Ohhhhhhh you can do it!!! You're right though, you DO have to be VERY careful and have lots of patience. Patience is something I am lacking in so God must have decided to put orchids in my life to teach me a few lessons......
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Old 02-13-2009, 06:48 PM
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Can I ask: What is the purpose of replating?
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Old 02-13-2009, 07:21 PM
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Doodlekitty - I think I will be sending the pod on Tuesday. I hope that has viable seeds. This should be fun.
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Old 02-14-2009, 01:29 PM
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Quote:
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Doodlekitty - I think I will be sending the pod on Tuesday. I hope that has viable seeds. This should be fun.
Oh Good!!! I JUST cooked up some media on the 11th and just waiting and watching for contamination (or NO contamination).
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Old 02-14-2009, 02:23 PM
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Can I ask: What is the purpose of replating?
The little protocorms are far too crowded to continue growing as originally plated. The second plating spreads them out a little more.

Doodlekitty! I'm so excited watching this thread!
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Old 02-14-2009, 05:46 PM
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Thank you Patti. I understand now.
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Old 02-15-2009, 08:43 AM
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Hi everyone,

I've been thinking about starting a new thread for our little pod project. What do you think of the title "The Orchid Geek Pod Project"?
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Old 02-15-2009, 08:48 AM
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Hi everyone,

I've been thinking about starting a new thread for our little pod project. What do you think of the title "The Orchid Geek Pod Project"?
That sounds good to me, doodles, but most of us are just watching! This is really cool!
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Old 02-17-2009, 06:39 PM
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I have some sad news. I was out of town over the weekend, went to Vegas, and upon my return the beautiful green pod turned to brown and released its seeds all over the surrounding pots.

I was going to ship it today, but I am too late. I tried to get the pod in a baggie, but there didn't seem to be any left. I guess I will have to try again next year.

The good news is that my Paph. lowii is in spike again. I will collect the pollen and try again when the Paph. primulinum spikes again. It has a growth that should bloom this year.

Sorry for creating a bunch of excitement over what appears to be nothing now.
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Old 02-17-2009, 06:46 PM
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No use crying over spilled seeds, I always say... Maybe we can all try pollinating, and see if we can come up with a pod for doodlekitty! Maybe it could be a project to grow the seedlings, (If doodlekitty were willing to get the seedlings that far!)
... Just a thought...
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Old 02-18-2009, 10:46 AM
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Don't you hate when that happens?? You wait and wait for months to catch it at the perfect time and then overnight "poof" it pops......just happened to me the other day with an Epi someone gave me.

No worries......and congrats on the new spike!!
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Old 02-18-2009, 10:58 AM
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When to pollinate

Is there an opportune time to polinate a blossom?
Fresh new bloom?
Open for a few weeks?
Near the end of the bloom?

I have phals in all of these stages and would like to try pollinating......

Al
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Old 02-18-2009, 01:22 PM
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I remember reading somewhere that when a pod is getting close, loosely wrap it with a soft paper, ie toilet paper, that way if the pod bursts, some of the seeds are contained in the paper.
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Old 02-20-2009, 11:03 PM
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Smile

A good trick I tend to do is loosely staple a coffee filter fast to the pod and fold the other part up to slide under the staple. Its my fail safe plan when I'm out of town. It creates an envelop to catch pollen, its breathable, and doesn't hold moisture real well inside. Cause if the pod splits and there is a high amount of moisture around the seed you will for sure have bacteria problems at the flasking level.

Green pod and documenting pod times greatly improves the lost of seed. Its harder to understand pod times for complex hybrids. But primary hybrids and species are a lot easier. Take note to the pod parent, and not the pollen parent, 9 times out of 10 the pod parent will influence the pod duration. The pollen parent usually only influences flower characteristics, and offers some traits that are only displayed after the flasking stage.

Creating hybrids is an exciting challenge. Just remember if you can picture the hybrid in your mind its probably not worth trying. Its the ones that you can't even picture that tend to turn into something special. I've done probably about 150 hybrids now some were pointless and lack a "wow factor." Others all I can say is "wow" when it first bloomed. Right there is the part that keeps everyone lured into this exciting concept.

Good luck to everyone.

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Old 02-21-2009, 07:01 PM
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Quote:
Originally Posted by Bird Song Farm View Post
Is there an opportune time to polinate a blossom?
Fresh new bloom?
Al
Any time before the flower is about to wither ? I do it all the time time from just
blooming to almost end of bloom.
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Old 02-21-2009, 11:36 PM
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I don't know what you are using for media, but I have a very good recipe that is inexpensive to produce if you are interested.
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Old 02-28-2009, 10:33 AM
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Hi Rod, thanks for the offer.....I'm open for all the help I can get.

My approach has been to try different media...I've purchased some from phytotech as well as the OBSP....I haven't been brave enough to brew up my own recipe. It was daunting enough just performing the flasking of my pods.
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Old 02-28-2009, 05:12 PM
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how are the little ones in the flask doing?
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Old 02-28-2009, 06:42 PM
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Growing like little weeds!! I've still got what seems like zillions in the mother flasks and they are MUCH smaller than the reflasked ones here. They seem to be getting lighter....(yellow?) I hope that is ok. So here are the "chids" now 3 months old!!!
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Old 02-28-2009, 11:07 PM
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so cool!!!!!
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Old 03-01-2009, 12:22 AM
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If anyone reading this thread is interested, here is a recipe I have had great success with.

10 gm agar (can be found in oriental markets)
6 cups distilled water
2 bananas for replate 1 for mother flask
2 tsp activated carbon (before grinding to powder)
8 drops of superthrive
1/4 tsp fertilizer 20-20-20
then add one drop of KLN root stimulator to each flask

will make 14 flasks with 3/4 cup media each
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Old 03-03-2009, 09:15 AM
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Thanks Rod! Is this a general flasking media or specific to mother flasks?
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