| Hi Joe,
From what you've told us, it looks like your sterilization procedures are the problem. It could also be that you're seed is so contaminated that is makes sterilizing nearly impossible.
What are you using to sterilize? Bleach? H2O2? I find bleach works good. Maybe up you concentration by 10% or increase the duration. Or do both. Do you rinse seed with sterile water?
Experiment! and keep records so you can duplicate the successful outcome.
Good luck and keep trying! |